Concomitant caries and calculus formation from dentin in situ caries model [version 1; referees: 2 approved]

The aim of this study was to test the possibility of the concomitant formation of calculus deposits and caries from dentin caries model for short time in situ periods. Six volunteers wore palatal removal appliances with four polished dentin specimens protected from intra-oral mechanical forces for up to 14 days. Each volunteer applied a 50% sucrose solution (four times a day) on the specimens and performed a daily mouthwash with 0.05% NaF. Samples were removed after 2, 5, 9 and 14 days . Demineralization was analyzed by in situ stereomicroscopy and SEM (secondary electrons and backscattered electrons modes) and calculus was analyzed by energy dispersive spectroscopy and fluorescence spectroscopy. Seventeen samples, at least one sample from each volunteer, presented dental calculus on both carious and non-carious ones, detected in all time intervals. Ca/P ratios of dental calculus ranged from 1.1 to 1.7. Some large calculus deposits on carious surfaces were confirmed by fluorescence. In conclusion, concomitant caries and calculus formation can be found in dentin caries formed . This has important repercussions on the in situ study of surface phenomena on the interface between hard dental tissues and dental plaque. Frederico B de Sousa ( ) Corresponding author: [email protected] de Sousa FB, Mangueira PJ, Tames DR How to cite this article: et al. Concomitant caries and calculus formation from dentin caries in situ 2013, :3 (doi: ) model [version 1; referees: 2 approved] F1000Research 2 10.12688/f1000research.2-3.v1 © 2013 de Sousa FB . This is an open access article distributed under the terms of the , Copyright: et al Creative Commons Attribution Licence which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Data associated with the article are available under the terms of the (CC0 1.0 Public domain dedication). Creative Commons Zero "No rights reserved" data waiver The first author thanks the Brazilian Ministry of Education (CAPES) for financial support (doctorate scholarship). The other Grant information: authors are grateful to CNPq Brazilian Federal Agency of Research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. No competing interests were disclosed. Competing interests: 04 Jan 2013, :3 (doi: ) First published: 2 10.12688/f1000research.2-3.v1 1 1 2 3